Comparison of the Efficiency to One-Step and Two-Step Anther Culture in Rice (Oryza sativa L)
Jeong Hoon Min, Jin Soo Sim, Hyun-Suk Lee, Young-Hie Park, Gyu-Hwan Park, Kyung-Min Kim

Anther culture method has commonly been performed two-step culture of callus induction during the plant regeneration stage. Two-step culture was generally required to a much longer duration of culture for regeneration. There are still needed to be solved the disadvantage of the two-step culture technique. Anther of F1 plants derived from Drimi2*3/AR1 of new plant type and improved rice cultivars were compared with method efficiency using one-step and two-step culture method. The results showed that one-step culture was more effective than two-step culture. Callus induction averaged 8.2% for one-step culture and 6.1% for two-step culture. The proportion of plants that survived after colchicine treatment was similar as 17.5% for one-step culture and 16.7% for two-step culture. However, there was a highly significant difference when results of plant regeneration were compared. Frequency of regenerated plant averaged 13.6% for one-step culture, which was 7.1% higher than two-step culture (6.5%). These results provide valuable data that may be used to establish an efficient plant regeneration system for rice cultures.

Full Text: PDF     DOI: 10.15640/jaes.v4n1a10