Organogenesis of Citrus Rootstocks Using Mature Explants
Thiresia-Teresa Tzatzani, Kortessa Dimassi, Ioannis Therios

Organogenesis of the commercial Citrus rootstocks P. trifoliata, C. aurantium and Swingle citrumelo was studied, using mature shoot and leaf explants. In P. trifoliata, the number of sprouted buds per shoot explant increased at both 0.5 and 2.0 mg L-1 BA concentrations; shoot number increased significantly at 2 mg L-l BA, while shoot length increased significantly at both BA concentrations. In C. aurantium explants, shoot number increased when 2.0 mg L-1 BA were added in the medium, and shoot length increased using 0.5 mg L-1 BA. In all the treatments of P. trifoliata and C. aurantium explants, callus formation was observed at their base. On leaf explants, it was observed callus formation of different colour; yellow, yellow - brown, brown and green. In C. aurantium the highest callus formation was observed using 5.500 mg L-1 TDZ. In P. trifoliata the highest percentage of callus formation were observed using 2.0 or 5.0 mg L-1 NAA, with 0.5 mg L-1 BA, and the callus was green. In Citrumelo leaf explants, TDZ caused the maximum callus formation, alone or in combination with BA. So, in vitro organogenesis of citrus rootstocks can be accomplished using mature tissues, in a successful and quick way.

Full Text: PDF     DOI: 10.15640/jaes.v7n1a2